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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been noticed in many tumors, which results in chromosome instability, and therefore could add to tumorigenesis. This protein was uncovered to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a important part in promoting cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are already described. Two further splice variants were reported but in depth nucleotide sequence information and facts will not be but offered.
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Blocking peptides are peptides that bind particularly into the focus on antibody and block antibody binding. These peptide ordinarily is made up of the epitope identified by the antibody.
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This manual outlines additional attainable optimization of cellular and immunoassay parameters to be certain the very best effects are...
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This guidebook outlines even further doable optimization of cellular and immunoassay parameters to make sure the best possible outcomes are...
Antibodies sure to the blocking peptide no more bind to the epitope within the target protein. By evaluating the staining from your blocked antibody as opposed to the antibody by itself, one can see which staining is unique.
Focus on Info Cyclin E belongs towards the really conserved cyclin household, whose members are characterised by a extraordinary periodicity in protein abundance throughout the mobile cycle. Cyclins functionality as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add to the temporal coordination of every mitotic event. Cyclin E varieties a fancy with and functions like a regulatory subunit of CDK2, whose exercise is required for cell cycle G1/S transition. This protein accumulates at the G1-S period boundary and is also degraded as cells development through S stage.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both antibodies...
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